How to perform the WATS3D Biopsy: a procedural tutorial

Preparing to Perform the WATS3D Biopsy

Preparation for Use

Before beginning the procedure, components of the WATS3D biopsy kit should be opened and laid out. The WATS3D instrument is passed down the biopsy channel of the endoscope.

Vimeo

WATS3D Procedure

With the tip of the brush advanced over the biopsy site, the brush is rotated and repeatedly passed back and forth until pinpoint bleeding is observed. The brush is retracted into the sheath and removed from the endoscope.

WATS3D Specimen Collection

Preserving Specimen

Cellular material from the brush is transferred to a slide and fixed; the brush tip is clipped into a vial. A second brush is used to re-brush the biopsy site and the brush tip is clipped into the vial.Read more

Procedural Tutorial Overview

  1. The WATS3D biopsy device is enclosed in a 2.5-mm sheath that is passed through the operating channel of a standard endoscope. During endoscopy procedure a WATS biopsy of the suspected area and/or squamocolumnar junction should be obtained.
  2. The WATS3D brush is passed down the biopsy channel. The tip should be advanced and placed against the surface of the mucosa. While maintaining firm pressure, the tip should be rotated and repeatedly passed back and forth over the abnormality and/or squamocolumnar junction until pinpoint bleeding is observed.
  3. Once the sample is collected, the WATS3D tip is again retracted into the surrounding sheath and the brush removed from channel. The cellular material is spread from the instrument onto the bar-coded side of the enclosed glass slide. The bristle portion of WATS3D is clipped off into the enclosed vial. The sample is then sent to the CDx for processing and analysis.
  4. A specimen smear and cellblock are prepared from the sample and analyzed with the aid of a proprietary high speed computer which localizes potentially abnormal cells and cell clusters through a combined morphological, molecular and DNA ploidy analysis. Potentially abnormal cells and cell clusters are selected by the computer for examination on a high resolution monitor by a specially trained pathologist who makes the final diagnosis.
“…WATS3D is actually a simple procedure – I introduce a brush into the working channel and do a circumferential sampling of the distal esophagus- it adds about a minute to my procedures and it’s very simple.”
Dr. Gina Sam, Director of the Gastrointestinal Motility Center, Stony Brook Medical Center