How to perform the WATS3D Biopsy: a procedural tutorial
Cellular material from the brush is transferred to a slide and fixed; the brush tip is clipped into a vial. A second brush is used to re-brush the biopsy site and the brush tip is clipped into the vial.Read more
All specimens should be labeled with the patient information and the test form should be completed. The kit box serves as a pre-paid mailer for return of the kit to CDx Diagnostics for analysis.
Procedural Tutorial Overview
- The WATS3D biopsy device is enclosed in a 2.5-mm sheath that is passed through the operating channel of a standard endoscope. During endoscopy procedure a WATS biopsy of the suspected area and/or squamocolumnar junction should be obtained.
- The WATS3D brush is passed down the biopsy channel. The tip should be advanced and placed against the surface of the mucosa. While maintaining firm pressure, the tip should be rotated and repeatedly passed back and forth over the abnormality and/or squamocolumnar junction until pinpoint bleeding is observed.
- Once the sample is collected, the WATS3D tip is again retracted into the surrounding sheath and the brush removed from channel. The cellular material is spread from the instrument onto the bar-coded side of the enclosed glass slide. The bristle portion of WATS3D is clipped off into the enclosed vial. The sample is then sent to the CDx for processing and analysis.
- A specimen smear and cellblock are prepared from the sample and analyzed with the aid of a proprietary high speed computer which localizes potentially abnormal cells and cell clusters through a combined morphological, molecular and DNA ploidy analysis. Potentially abnormal cells and cell clusters are selected by the computer for examination on a high resolution monitor by a specially trained pathologist who makes the final diagnosis.
“…WATS3D is actually a simple procedure – I introduce a brush into the working channel and do a circumferential sampling of the distal esophagus- it adds about a minute to my procedures and it’s very simple.”